Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
Identifieur interne : 000846 ( Main/Exploration ); précédent : 000845; suivant : 000847Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
Auteurs : Valeria E. Perotti [Argentine] ; Alejandra S. Moreno [Argentine] ; Karina Tripodi [Argentine] ; Hernán A. Del Vecchio [Argentine] ; Guillermo Meier [Argentine] ; Fernando Bello [Argentine] ; Mariángeles Cocco [Argentine] ; Daniel Vazquez [Argentine] ; Florencio E. Podesta [Argentine]Source :
- Postharvest biology and technology [ 0925-5214 ] ; 2015.
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Abstract
Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.
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Perotti</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531</s1><s2>2000 Rosario</s2><s3>ARG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>2000 Rosario</wicri:noRegion></affiliation></author><author><name sortKey="Moreno, Alejandra S" sort="Moreno, Alejandra S" uniqKey="Moreno A" first="Alejandra S." last="Moreno">Alejandra S. Moreno</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531</s1><s2>2000 Rosario</s2><s3>ARG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>2000 Rosario</wicri:noRegion></affiliation></author><author><name sortKey="Tripodi, Karina" sort="Tripodi, Karina" uniqKey="Tripodi K" first="Karina" last="Tripodi">Karina Tripodi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531</s1><s2>2000 Rosario</s2><s3>ARG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>2000 Rosario</wicri:noRegion></affiliation></author><author><name sortKey="Del Vecchio, Hernan A" sort="Del Vecchio, Hernan A" uniqKey="Del Vecchio H" first="Hernán A." last="Del Vecchio">Hernán A. Del Vecchio</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531</s1><s2>2000 Rosario</s2><s3>ARG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>2000 Rosario</wicri:noRegion></affiliation></author><author><name sortKey="Meier, Guillermo" sort="Meier, Guillermo" uniqKey="Meier G" first="Guillermo" last="Meier">Guillermo Meier</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Estación Experimental Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA)</s1><s2>Colonia Yeruá</s2><s3>ARG</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>Colonia Yeruá</wicri:noRegion></affiliation></author><author><name sortKey="Bello, Fernando" sort="Bello, Fernando" uniqKey="Bello F" first="Fernando" last="Bello">Fernando Bello</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Estación Experimental Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA)</s1><s2>Colonia Yeruá</s2><s3>ARG</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>Colonia Yeruá</wicri:noRegion></affiliation></author><author><name sortKey="Cocco, Mariangeles" sort="Cocco, Mariangeles" uniqKey="Cocco M" first="Mariángeles" last="Cocco">Mariángeles Cocco</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Estación Experimental Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA)</s1><s2>Colonia Yeruá</s2><s3>ARG</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>Colonia Yeruá</wicri:noRegion></affiliation></author><author><name sortKey="Vazquez, Daniel" sort="Vazquez, Daniel" uniqKey="Vazquez D" first="Daniel" last="Vazquez">Daniel Vazquez</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Estación Experimental Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA)</s1><s2>Colonia Yeruá</s2><s3>ARG</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>Colonia Yeruá</wicri:noRegion></affiliation></author><author><name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E." last="Podesta">Florencio E. Podesta</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531</s1><s2>2000 Rosario</s2><s3>ARG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Argentine</country><wicri:noRegion>2000 Rosario</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Postharvest biology and technology</title><title level="j" type="abbreviated">Postharvest biol. technol.</title><idno type="ISSN">0925-5214</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Postharvest biology and technology</title><title level="j" type="abbreviated">Postharvest biol. technol.</title><idno type="ISSN">0925-5214</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biochemical analysis</term><term>Biochemistry</term><term>Citrus fruit</term><term>Citrus sinensis</term><term>Food preservation</term><term>Fruit</term><term>Glycolysis</term><term>Heat treatment</term><term>Orange</term><term>Postharvest</term><term>Proteomics</term><term>Refrigerated storage</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse biochimique</term><term>Traitement thermique</term><term>Citrus sinensis</term><term>Orange</term><term>Après récolte</term><term>Entreposage frigorifique</term><term>Agrume</term><term>Glycolyse</term><term>Fruit</term><term>Biochimie</term><term>Protéomique</term><term>Conservation aliment</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Agrume</term><term>Fruit</term><term>Biochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.</div></front></TEI><affiliations><list><country><li>Argentine</li></country></list><tree><country name="Argentine"><noRegion><name sortKey="Perotti, Valeria E" sort="Perotti, Valeria E" uniqKey="Perotti V" first="Valeria E." last="Perotti">Valeria E. Perotti</name></noRegion><name sortKey="Bello, Fernando" sort="Bello, Fernando" uniqKey="Bello F" first="Fernando" last="Bello">Fernando Bello</name><name sortKey="Cocco, Mariangeles" sort="Cocco, Mariangeles" uniqKey="Cocco M" first="Mariángeles" last="Cocco">Mariángeles Cocco</name><name sortKey="Del Vecchio, Hernan A" sort="Del Vecchio, Hernan A" uniqKey="Del Vecchio H" first="Hernán A." last="Del Vecchio">Hernán A. Del Vecchio</name><name sortKey="Meier, Guillermo" sort="Meier, Guillermo" uniqKey="Meier G" first="Guillermo" last="Meier">Guillermo Meier</name><name sortKey="Moreno, Alejandra S" sort="Moreno, Alejandra S" uniqKey="Moreno A" first="Alejandra S." last="Moreno">Alejandra S. Moreno</name><name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E." last="Podesta">Florencio E. Podesta</name><name sortKey="Tripodi, Karina" sort="Tripodi, Karina" uniqKey="Tripodi K" first="Karina" last="Tripodi">Karina Tripodi</name><name sortKey="Vazquez, Daniel" sort="Vazquez, Daniel" uniqKey="Vazquez D" first="Daniel" last="Vazquez">Daniel Vazquez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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